张 杉 （1996.04）
博士 讲师
E-mail：zhangshan@hbnu.edu.cn/zhangs.0422@qq.com
个人简历
■教育经历：
2021-2025年， 武汉大学 分析化学专业，获理学博士学位
2018-2021年，华中农业大学 生物工程专业，获工程硕士专业学位
2014-2018年，廊坊师范学院 生物科学专业，获理学学士学位
■工作经历：
2025.7-今，湖北师范大学生命科学学院任教
研究领域
主要从事于生物分析化学和核酸表观遗传修饰分析领域的研究。具体研究领域包括：
■核酸表观遗传修饰分析方法的开发及应用。
■蓝藻对环境因素适应性表观遗传机制的探索。
■可食用蓝藻的表观遗传驯化和育种改良。
■核酸编辑和修饰工具的开发及应用。
代表性成果
■科研论文：
Zhang, S#.; Liang, Y#.; Feng, T.; Guo, X.; Wang, M.; Ji, T. T.; Xiong, J.; Xiao, X.; Liu, Y.; Liu, Y. B.; Ci, W. M*.; Xie, N. B*.; Yuan, B. F*. Whole-genome mapping of 5-hydroxymethylcytosine at single-base resolution by double-stranded DNA deamination sequencing. CCS Chem. 2025, DOI: 10.31635/ccschem.025.202405023.
Zhang, S#.; Xie, N. B#.; Zeng, L.; Gang, F. Y.; Gu, Y. H.; Wang, M.; Guo, X.; Ji, T. T.; Xiong, J*.; Yuan, B. F*. Direct sequencing of DNA 5-methylcytosine by engineered dioxygenase NTET-assisted eNAPS. Chem Sci. 2025, 16 (32), 14800-14810.
You, X. J#.; Zhang, S#.; Chen, J. J.; Tang, F.; He, J.; Wang, J.; Qi, C. B.; Feng, Y. Q.; Yuan, B. F*. Formation and removal of 1, N6-dimethyladenosine in mammalian transfer RNA. Nucleic Acids Res. 2022, 50 (17), 9858-9872. (Co-first author).
Liu, W#.; Ma, Z. C#.; Zhang, S.; Gang, F. Y.; Ji, T. T.; Gu, Y. H.; Xie, N. B.; Gu, S. Y.; Guo, X.; Feng, T.; Liu, Y*.; Xiong, J*.; Yuan, B. F*. Direct Single-Nucleotide Resolution Sequencing of DNA 5-Methylcytosine Using Engineered DNA Methyltransferase-Mediated CMD-seq. Chem Sci. 2025, 16(20), 8788-8799.
Hao, Y#.; Ji, T. T#.; Gu. S. Y#.; Zhang, S.; Gu, Y. H.; Guo, X.; Zeng, L.; Gang, F. Y.; Xiong, J.; Feng, Y. Q*.; Xie, N. B*.; Yuan, B. F*. (2025). Deaminase-Driven Random Mutation Enables Efficient DNA Mutagenesis for Protein Evolution. Chem Sci. 2025, 16(20), 8752-8763.
Wang, M#.; Xie, N. B#.; Gang, F. Y.; Zhang, S.; Zeng, L.; Ji, T. T.; Xiong, J.; Guo, X.; Hao, Y.; Yuan, B. F*. Bisulfite-Free Whole-Genome Mapping of 5-Methylcytosine at Single-Base Resolution by NTD-seq. Sci China Life Sci. 2025, DOI: 10.1007/s11427-024-2702-8.
Zeng, L#.; Gang, F. Y#.; Ji, T. T#.; Zhang, S.; Guo, X.; Hao, Y.; Xiong, J.; Wei, Z. W*.; Xie, N. B*.; Yuan, B. F*. Precise recognition and quantification of locus-specific DNA methylation using engineered ROS1. Chin J Chem. 2025, DOI: 10.1002/cjoc.70044.
Ji, T. T#.; Wang, M#.; Guo, X.; Gang, F. Y.; Zhang, S.; Xiong, J.; Gu. Y. H*.; Xie. N. B*.; Yuan, B. F*. Glycosidase-Enabled Locus-Specific Detection of 8-Oxo-7,8-dihydroguanine in Genomes under Oxidative Stress. Anal Chem. 2025, 97(15), 8564-8573.
Guo, X#.; Wu, J#.; Ji, T. T.; Wang, M.; Zhang, S.; Xiong, J.; Gang, F. Y.; Liu, W.; Gu, Y. H.; Liu, Y*.; Xie, N. B*.; Yuan, B. F*. Orthologous mammalian A3A-mediated single-nucleotide resolution sequencing of DNA epigenetic modification 5-hydroxymethylcytosine. Chem Sci. 2025, 16 (9), 3953-3963.
Gang, F. Y#.; Xie, N. B#.; Wang, M.; Zhang, S.; Ji, T. T.; Liu, W.; Guo, X.; Gu, S. Y.; Yuan, B. F*. Bisulfite-Free and Quantitative Detection of DNA Methylation at Single-Base Resolution by eROS1-seq. Anal Chem. 2024, 96 (52), 20559-20567.
Min, Y. H#.; Shao, W. X#.; Hu, Q. S#.; Xie, N. B.; Zhang, S.; Feng, Y. Q.; Xing, X. W.; Yuan, B. F*. Simultaneous Detection of Adenosine-to-Inosine Editing and N6-Methyladenosine at Identical RNA Sites through Deamination-Assisted Reverse Transcription Stalling. Anal Chem. 2024, 96 (21), 8730-8739.
Guo, X#.; Xie, N. B#.; Chen, W#.; Ji, T. T.; Xiong, J.; Feng, T.; Wang, M.; Zhang, S.; Gu, S. Y.; Feng, Y. Q*.; Yuan, B. F*. AlkB-Facilitated Demethylation Enables Quantitative and Site-Specific Detection of Dual Methylation of Adenosine in RNA. Anal Chem. 2024, 96 (2), 847-855.
Shao, W. X#.; Min, Y. H#.; Chen, W#.; Xiong, J.; Guo, X.; Xie, N. B.; Zhang, S.; Yu, S. Y.; Xie, C.; Feng, Y. Q.; Yuan, B. F*. Single-Base Resolution Detection of N6-Methyladenosine in RNA by Adenosine Deamination Sequencing. Anal Chem. 2023, 95 (28), 10588-10594.
■发明专利：
袁必锋，张杉。双链胞嘧啶脱氨酶在DNA中5hmC修饰单碱基分辨率定位分析中的应用。申请号：202411769828.3，申请日期：2024.12.04。